This dialog can find restriction enzymes that cut in a marked DNA sequence (context menu Selection/Show enzymes that cut here in the DNA module). It can also find alternate versions of the DNA which will translate into the same amino acid sequence, but contains a new restriction site (silent mutation). A chosen enzyme/mutation will appear in the sequence (DNA or primer, respectively) upon OK.
The results can be changed by
- changing which enzyme group to search
- limiting the number of times an enzyme may cut in the whole sequence
- limiting the number of mutations needed for a restriction site to manifest (PCR module only)