GENtle/DNA markers

From Wikibooks, open books for an open world
Jump to navigation Jump to search
The GENtle manual

About - FAQ - Setup - DNA - Protein - PCR and Primer Design - Sequencing - Alignments - Calculators - Virtual Gel - Image Viewer - Tools and Dialogs - Web interface - Graphs and plots - DNA map - Sequence map - Dot plot - Restriction Identifier

This file contains DNA markers for virtual gels. You can add your own in the following form:

Name:amount,bp:amount,bp:amount,bp:amount,bp: ...
*Name is the name of the marker.
*bp is the number of base pairs in that band.
*amount is the amount of DNA in that band if 500 ng of the marker is loaded per lane (ng).
":amount" can be omitted. The default amount (20) is then used.

Markers you enter will be used in the next GENtle release!

Promega 1kb DNA Ladder: 60,10000:60,8000:6000:5000:4000:60,3000:2500:2000:1500:60,1000:750:500:253:250
GeneRuler 50 bp DNA Ladder:20,1031:73,900:64,800:57,700:50,600:43,500:71,400:28,300:21,250:18,200:28,150:11,100:21,50
GeneRuler DNA Ladder Mix:20,10000:11,8000:13,6000:16,5000:16,4000:22,3500:27,3000:69,2500:18,2000:46,1500:24,1200:16,1031:30,900:21,800:19,700:16,600:14,500:42,400:20,300:20,200:20,100
GeneRuler and O'GeneRuler 1 kb DNA Ladder:
GeneRuler and O'GeneRuler 1 kb Plus DNA Ladder:
GeneRuler and O'GeneRuler 100 bp DNA Ladder:
GeneRuler and O'GeneRuler 100 bp Plus DNA Ladder:
GeneRuler and O'GeneRuler Ultra Low Range DNA Ladder:
GeneRuler and O'GeneRuler Low Range DNA Ladder:
GeneRuler and O'GeneRuler Express DNA Ladder:
GeneRuler High Range DNA Ladder:
Lambda DNA/Eco130I (StyI) Marker:199,19329:80,7743:64,6223:44,4254:36,3472:28,2690:19,1882:15,1489:10,925:4,421:1,74
Lambda DNA/EcoRI+HindIII Mix:0.5,21226:219,5148:53,4973:51,4268:44,3530:36,2027:21,1904:19,1584:16,1375:14,947:10,831:8,564
Lambda DNA/HindIII Marker:238,23130:97,9416:68,6557:45,4361:24,2322:21,2027:6,564:1,125
Lambda DNA/Pst1 Marker:0.5,11501:118.6,5077:52.3,4749:49,4507:46.5,2838:29.3,2556:26.3,2459:25.3,2443:25.2,2140:22.1,1986:20.5,1700:17.5,1159:11.9,1093:11.3,805:8.3,514:5.3,468:4.8,448:4.6,339:3.5,264:2.7,247:2.5
MassRuler High Range:20,10000:200,8000:160,6000:120,5000:100,4000:80,3000:60,2500:52,2000:40,1500
MassRuler Low Range:20,1031:200,900:180,800:160,700:140,600:120,500:200,400:80,300:60,200:40,100:20,80
MassRuler Mix:20,10000:200,8000:160,6000:120,5000:100,4000:80,3000:60,2500:50,2000:40,1500:32,1031:200,900:180,800:160,700:140,600:120,500:200,400:80,300:60,200:40,100:20,80
pBR322 DNA/AluI Marker:104,908:76,659:75,656:60,521:46,403:32,281:30,257:26,226:12,100:10, 90:1,63:1,57:1,49:1,46:1,19:1,15:1,11
Plasmid Factory 1kb DNA Ladder:115,10000:92,8000:69,6000:57,5000:46,4000:35,3000:29,2500:23,2000:17,1500:12,1000:6,500
Stratagene 1kb DNA Ladder:50,12000:50,10000:50,9000:50,8000:50,7000:40,6000:42,5000:42,4000:43,3000:40,2000:10,1500:8,1000:8,750:7,500:10,250
NEB 1kb DNA Ladder:42,10002:42,8001:50,6001:42,5001:33,4001:125,3001:48,2000:36,1500:42,1000:21,517:21,500
NEB 100bp DNA Ladder:45,1517:35,1200:95,1000:27,900:24,800:21,700:18,600:97,500:97,517:38,400:29,300:25,200:48,100