Structural Biochemistry/AgarACE ®, Enzyme
AgarACE™ Enzyme(a) is an agarose-digesting enzyme which is used for simple and quantitative recovery of DNA or RNA from agarose gels. This enzyme is thermostable in agarose gel procedures. It can hydrolyze 200mg of 1% LMP agarose in 15 minutes. Good recovery is possible for DNA between 300-3,000 bp. There is no buffer required. This enzyme is used for cloning, ligation, primer labeling, PCR, and sequencing. It has also been suggested that it can give high yields of "translated protein product in reticulocyte lysate translation systems" in isolated RNA. The quality of the nucleic acids after undergoing electrophoresis is not well known.  AgarACE™ Enzyme was developed by the company, Promega. The creation of this enzyme caused certain protocols to become easier and produce faster results.
References[edit | edit source]
- Knuth, Mark, Kimberly Knoche, and Paul Otto. "AgarACE™ Agarose-Digesting Enzyme Part Three: DNA/RNA Recovery and Applications Testing for Use in Translation, Transcription/Translation, Prime-a-Gene® Labeling and Fluorescent DNA Sequencing." Promega Notes Magazine. Number 61, 1997, p.21.