Proteomics/Protein Separations- Electrophoresis/History of Electrophoresis
History of Electrophoresis
1930s First reports of the use of sucrose for gel electrophoresis.
1937 The official launching of Tiselius' moving boundary electrophoresis (MBE).
1939 Zone Electrophoresi
1950 Rich in introducing novel methodologies in zone electrophoresis (ZE)
1950 Agar Gel electrophoresis
1955 Introduction of starch gels, not very good separation
1957 Cellulose acetate Electrophoresis(CAc) by Kohn
1959 Introduction of acrylamide gels for 1st time (Raymond and Winstraub) Accuracy of control of parameters such as pore size and stability
1961 Electrophoresis in silica gel by Honnegar
1961IsoElectricFocusing by Svensson
1964 Electrophoresis in thin layer of sephadex beads by Fasella et.al
1964 Disc gel electrophoresis Ornstein and Davis
1969 Introduction of denaturing agents especially SDS separation of protein subunit (Beber and Osborn)
1970 Laemmli-T4 phage separated 28 components Mstacking gel + SDS
1975 2 Dimensional gels OFarrell isoelectric focussing then SDS gel electrophoresis.
1977 Sequencing gels
Late 1970s agarose gels
1980sImmobilized pH gradients
1983 Pulsed field gel electrophoresis
1983 Capillary electrophoresis introduced
Electrophoresis: the march of pennies, the march of dimes. Righetti PG.