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Cultured embryonal carcinoma were stained with either DiI (red) or DiO (green). The image was captured by scanning confocal microscopy.
DiI is a hydrophobic and lipophilic cyaninedye used for scientific purposes, such as single molecule imaging, Fate mapping, and neuronal tracing (as it is retained in the lipid bilayers). Its chemical name is 1,1'-dioctadecyl-3,3,3'3'-tetramethylindocarbocyanine perchlorate, the chemical formula is C59H89ClN2O4