Proteomics/Protein Separations- Electrophoresis/Timeline of Electrophoresis

1. Introduction to Electrophoresis
2. Gel Electrophoresis
3. One Dimentional Gel Electrophoresis
4. Two Dimensional Polyacrylamide Gel Electrophoresis(2D-PAGE)
5. Differential in Gel Electrophoresis(DIGE)
6. QPNC-PAGE
7. Capillary Electrophoresis
8. Timeline of Electrophoresis
9. Databases
10. Web Pages
11. Online Applications
12. Further Readings

Timeline of Electrophoresis

1930s : Use of sucrose for gel electrophoresis.

1937  : The official launching of Tiselius' moving boundary electrophoresis (MBE).

1939  : Zone Electrophoresis

1950  : Rich in introducing novel methodologies in zone electrophoresis (ZE)

1950  : Agar Gel electrophoresis

1955  : Introduction of starch gels

1957  : Cellulose acetate Electrophoresis(CAc) by Kohn

1959  : Arrival of Acrylamide gels (Raymond and Winstraub)

1961  : Electrophoresis in silica gel by Honnegar

1961  : IsoElectricFocusing by Svensson

1964  : Electrophoresis in thin layer of sephadex beads by Fasella et.al

1964  : Ornstein and Davis introduced Disc-Gel Electrophoresis.

1969  : Beber and Osborn introduced denaturing agents i.e. SDS separation of protein subunit

1970  : Stacking gel and SDS by Laemmli was used to separate T4 phage proteins

1975  : 2-D gels :Isoelectric focussing and SDS gel electrophoresis by OFarrell.

1977  : Arrival of Sequencing gels

1970s : Arrival of Agarose gels

1980s : Immobilized pH gradients

1983  : Pulsed field gel electrophoresis was invented

1983  : Introduction of Capillary electrophoresis

Next Section Databases

References

• http://www.csupomona.edu/~nebuckley/Courses/Bio451/Lectures/Bio451Lecture4.htm

Subcription Based References

• Righetti PG, Electrophoresis: the march of pennies, the march of dimes; Journal of Chromatography A. 2005 Jun 24;1079(1-2):24-40. Abstract[[1]]