Chemical Sciences: A Manual for CSIR-UGC National Eligibility Test for Lectureship and JRF/Capillary electrophoresis-mass spectrometry

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Capillary electrophoresis mass spectrometry (CEMS) is an analytical chemistry technique formed by the combination of the liquid separation process of capillary electrophoresis with mass spectrometry.[1] Ions are typically formed by electrospray ionization,[2] but they can also be formed by matrix-assisted laser desorption ionization[3] or other ionization techniques. It has applications in basic research in proteomics[4] and quantitative analysis of biomolecules[5] as well as in clinical medicine.[6][7]


  1. Loo JA, Udseth HR, Smith RD (June 1989). "Peptide and protein analysis by electrospray ionization-mass spectrometry and capillary electrophoresis-mass spectrometry". Anal. Biochem. 179 (2): 404–12. doi:10.1016/0003-2697(89)90153-X. PMID 2774189. 
  2. Maxwell EJ, Chen DD (October 2008). "Twenty years of interface development for capillary electrophoresis-electrospray ionization-mass spectrometry". Anal. Chim. Acta 627 (1): 25–33. doi:10.1016/j.aca.2008.06.034. PMID 18790125. 
  3. Zhang H, Caprioli RM (September 1996). "Capillary electrophoresis combined with matrix-assisted laser desorption/ionization mass spectrometry; continuous sample deposition on a matrix-precoated membrane target". J Mass Spectrom 31 (9): 1039–46. doi:10.1002/(SICI)1096-9888(199609)31:9<1039::AID-JMS398>3.0.CO;2-F. PMID 8831154. 
  4. Metzger J, Schanstra JP, Mischak H (August 2008). "Capillary electrophoresis-mass spectrometry in urinary proteome analysis: current applications and future developments". Anal Bioanal Chem 393 (5): 1431–42. doi:10.1007/s00216-008-2309-0. PMID 18704377. 
  5. Ohnesorge J, Neusüss C, Wätzig H (November 2005). "Quantitation in capillary electrophoresis-mass spectrometry". Electrophoresis 26 (21): 3973–87. doi:10.1002/elps.200500398. PMID 16252322. 
  6. Kolch W, Neusüss C, Pelzing M, Mischak H (2005). "Capillary electrophoresis-mass spectrometry as a powerful tool in clinical diagnosis and biomarker discovery". Mass Spectrom Rev 24 (6): 959–77. doi:10.1002/mas.20051. PMID 15747373. 
  7. Dakna M, He Z, Yu WC, Mischak H, Kolch W (November 2008). "Technical, bioinformatical and statistical aspects of liquid chromatography-mass spectrometry (LC-MS) and capillary electrophoresis-mass spectrometry (CE-MS) based clinical proteomics: A critical assessment". J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 877 (13): 1250–8. doi:10.1016/j.jchromb.2008.10.048. PMID 19010091.