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Summary

Real time quantitative PCR using TaqMan^® probes. (1) In intact TaqMan^® probes, reporter fluorescence is quenched due to FRET. (2) Probes and the complementary DNA strand are hybridized and reporter fluorescence is still quenched. (3) During PCR, the 5'-ends of the probe are degraded by the Taq polymerase. The fluorescent reporter is released.

This image was created by S. Jähnichen using 3d studio max and Photoshop Elements.

Licensing

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