Metabolomics/Analytical Methods/Sample Preparation

From Wikibooks, open books for an open world
Jump to navigation Jump to search

Back to Previous Chapter: Hormones
Next chapter: Computational Modeling of Metabolic Control
Go to Fluids
Go back to Mass Spectrometry

Sample Preparation[edit | edit source]

In the steps leading up to chemical analytical methods such as NMR and mass spec, a sample must be first prepared to obtain the metabolites.

Articles and Web Pages for Review and Inclusion[edit | edit source]

Peer-Reviewed Article #1:[edit | edit source]

Analytical strategies for LC-MS-based targeted metabolomics

J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Aug 15;871(2):236-42. Epub 2008 Apr 29'"

Main Focus[edit | edit source]

Identify the main focus of the resource. Possible answers include specific organisms, database design, intergration of information, but there are many more possibilities as well.

New Terms[edit | edit source]

New Term 1
Definition. (source: http://)
New Term 2
Definition. (source: http://)
New Term 3
Definition. (source: http://)
New Term 4
Definition. (source: http://)
New Term 5
Definition. (source: http://)
New Term 6
Definition. (source: http://)
New Term 7
Definition. (source: http://)
New Term 8
Definition. (source: http://)
New Term 9
Definition. (source: http://)
New Term 10
Definition. (source: http://)

Summary[edit | edit source]

Enter your article summary here. Please note that the punctuation is critical at the start (and sometimes at the end) of each entry. It should be 300-500 words. What are the main points of the article? What questions were they trying to answer? Did they find a clear answer? If so, what was it? If not, what did they find or what ideas are in tension in their findings?

Relevance to a Traditional Metabolism Course[edit | edit source]

Enter a 100-150 word description of how the material in this article connects to a traditional metabolism course. Does the article relate to particular pathways (e.g., glycolysis, the citric acid cycle, steroid synthesis, etc.) or to regulatory mechanisms, energetics, location, integration of pathways? Does it talk about new analytical approaches or ideas? Does the article show connections to the human genome project (or other genome projects)?

Peer-Reviewed Article #2:[edit | edit source]

Tissue fractionation by hydrostatic pressure cycling technology: the unified sample preparation technique for systems biology studies

J Biomol Tech. 2008 Jul;19(3):189-99.'"

Main Focus[edit | edit source]

Identify the main focus of the resource. Possible answers include specific organisms, database design, intergration of information, but there are many more possibilities as well.

New Terms[edit | edit source]

New Term 1
Definition. (source: http://)
New Term 2
Definition. (source: http://)
New Term 3
Definition. (source: http://)
New Term 4
Definition. (source: http://)
New Term 5
Definition. (source: http://)
New Term 6
Definition. (source: http://)
New Term 7
Definition. (source: http://)
New Term 8
Definition. (source: http://)
New Term 9
Definition. (source: http://)
New Term 10
Definition. (source: http://)

Summary[edit | edit source]

Enter your article summary here. Please note that the punctuation is critical at the start (and sometimes at the end) of each entry. It should be 300-500 words. What are the main points of the article? What questions were they trying to answer? Did they find a clear answer? If so, what was it? If not, what did they find or what ideas are in tension in their findings?

Relevance to a Traditional Metabolism Course[edit | edit source]

Enter a 100-150 word description of how the material in this article connects to a traditional metabolism course. Does the article relate to particular pathways (e.g., glycolysis, the citric acid cycle, steroid synthesis, etc.) or to regulatory mechanisms, energetics, location, integration of pathways? Does it talk about new analytical approaches or ideas? Does the article show connections to the human genome project (or other genome projects)?