GENtle/Silent Mutagenesis

From Wikibooks, open books for an open world
< GENtle
Jump to: navigation, search
GENtle splash screen.png GENtle: Tools and Dialogs

Ligation - Options - Databases - Import - Enter sequence - Sequence editor - Restriction Assistant - PCR troubleshoot dialog - Proteolysis assistant - Projects - Edit primer dialog - Find dialog - Printing - Enzyme management - Sequencing Primers - Silent Mutagenesis - Automatic annotation

The silent mutation dialog

This dialog can find restriction enzymes that cut in a marked DNA sequence (context menu Selection/Show enzymes that cut here in the DNA module). It can also find alternate versions of the DNA which will translate into the same amino acid sequence, but contains a new restriction site (silent mutation). A chosen enzyme/mutation will appear in the sequence (DNA or primer, respectively) upon OK.

The results can be changed by

  • changing which enzyme group to search
  • limiting the number of times an enzyme may cut in the whole sequence
  • limiting the number of mutations needed for a restriction site to manifest (PCR module only)