Summary

DescriptionSingle YFP molecule superresolution microscopy.png	English: Superresolution Microscopy/ Optical nanoscopy using SPDMphymod as a new localization microscopy technique for standard fluorescent dyes SPDMphymod image of protein distribution in a human cancer cell. Enlarged inserts above and below: Positions of single fluorescent molecules in Gaussian intensity shape of 5 nm standard deviation. Typical distance measurements in the 15 nm range are highlighted Fundamental to SPDMphymod are blinking phenomena (flashes of fluorescence), induced by reversible bleaches (metastable dark states). Individual molecules of the same spectral emission color can be detected. Counting individual molecules up to a density of 1000/µm2 – at present, this is possible in an area of up to 5000 µm2. Christoph Cremer, emeritus at Heidelberg university [1] Basic publications: Lemmer P, Gunkel M, Baddeley D, Kaufmann R, Urich A, Weiland Y, Reymann J, Müller P, Hausmann M, Cremer C: SPDM – Light Microscopy with Single Molecule Resolution at the Nanoscale. In: Applied Physics B 2008; 93: 1–12 Manuel Gunkel, Fabian Erdel, Karsten Rippe, Paul Lemmer, Rainer Kaufmann, Christoph Hörmann, Roman Amberger and Christoph Cremer: Dual color localization microscopy of cellular nanostructures. In: Biotechnology Journal, 2009, 4, 927-938. ISSN 1860-6768
Date	1 December 2009
Source	Own work
Author	Andy Nestl
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Other versions	[edit] English Russian

Gallery

• Super Resolution Microscopy - Localisation Microscopy
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  Breast Cancer Cells: 3D Dual Color Super Resolution Microscopy of Her2 and Her3 & cluster calculations
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  Single YFP molecule detection in a human cancer cell. Typical distance measurements 15 nm
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  Co- localisation microscopy with GFP and RFP fusion proteins (nucleus of a bone cancer cell) 120.000 localized molecules in a widefield area(470 µm2)
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  Label-free Localisation Microscopy SPDM - Super Resolution Microscopy reveals prior undetebable intracellular structures
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  Investigation of human eye tissue, affected by macular degeneration AMD
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  Virus Super Resolution Microscopy SPDM Cremer/Wege labs

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Original upload log

This image was originally uploaded in BMP format as File:Single_YFP_molecule_superresolution_microscopy.tif. The original upload history (excluding reverts) is shown below:

• 2009-12-01T12:00:38Z Andy Nestl 340x686 (699774 Bytes, image/x-bmp) test2
• 2009-12-01T11:45:07Z Andy Nestl 340x686 (700634 Bytes, image/tiff) {{Information |Description={{en|1=Superresolution Microscopy using SPDMphymod as a new localization microscopy technique for standard fluorescent dyes}} |Source={{own}} |Author=Andy Nestl |Date=2009-12-01 |Permission=given by Christoph

1. ↑ https://www.physik.uni-heidelberg.de/personen/lsf.php?details=1537 |titel=Fakultät für Physik und Astronomie |abruf=2020-10-01