Chemical Sciences: A Manual for CSIR-UGC National Eligibility Test for Lectureship and JRF/Triple quadrupole mass spectrometer
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A triple quadrupole mass spectrometer is a tandem mass spectrometer consisting of two quadrupole mass spectrometers in series, with a (non mass-resolving) radio frequency (RF) only quadrupole between them to act as a collision cell for collision-induced dissociation. The first (Q1) and third (Q3) quadrupoles serve as mass filters, whereas the middle (q2) quadrupole serves as a collision cell. This collision cell is an RF only quadrupole (non-mass filtering) using an inert gas such as Ar, He or N2 gas to provide collision-induced dissociation of a selected precursor ion that is selected in Q1. Subsequent fragments are passed through to Q3 where they may be filtered or scanned.
Structural mass spectrometry[edit | edit source]
This alignment of two mass spectrometers in succession with a collision cell (e.g. a collision quadrupole) in between allows structural mass spectrometry to be performed. For example, the Q1 may be set to select an ion of a known mass, which is fragmented in q2. The third quadrupole (Q3) is then set to scan the entire m/z range, giving information on the sizes of the fragments made. From the ion fragmentation information, the structure of the original ion can be deduced.
History[edit | edit source]
The arrangement of three quadrupoles was first developed by Morrizon of LaTrobe University, Australia for the purpose of studying the photodissociation of gas-phase ions.[1] The first triple-quadrupole mass spectrometer was developed at Michigan State University by Enke and Yost in the late 1970's.[2]
References[edit | edit source]
- ↑ Morrison, J. D. (1991), "Personal reminiscences of forty years of mass spectrometry in Australia", Organic Mass Spectrometry, 26: 183, doi:10.1002/oms.1210260404
- ↑ Yost, R. A.; Enke, C. G. (1978), "Selected ion fragmentation with a tandem quadrupole mass spectrometer" (PDF), Journal of the American Chemical Society, 100: 2274, doi:10.1021/ja00475a072