AP Biology/LABORATORY 1. Diffusion and Osmosis

From Wikibooks, the open-content textbooks collection

Jump to: navigation, search

[edit] Introduction

Low and high entropy. For any state of any system there is a number that describes how messy it is, this number is called entropy. Any spontaneous process will increase a system's entropy (as stated by the second law).
Diffusion
The random movement of molecules from an area of higher concentration of those molecules to an area of lower concentration.
Osmosis
the diffusion of water through a semi-permeable membrane from a region of higher water potential to a region of lower water potential.
Water Potential
is the potential energy of water in proportion to pure water. It is defined by the formula Ψ = Ψp + Ψs where
  • Ψ is the total water potential
  • Ψs is the solute potential of the solution
  • Ψp is the pressure potential of the solution

Osmosis and diffusion are two of the most important processes in the study of how organisms maintain homeostasis, particularly with regard to their electrolyte and water balances. This AP lab attempts to demonstrate the process of osmosis using dialysis bags.


[edit] Expected Results and Analysis

From only having glucose and starch within the dialysis bag, the glucose diffuses and there is a presence of glucose inside the cup that was initially distilled water and the absence of starch. This is because the selectively permeable dialysis bag, cell membrane, only allows small molecules, such as glucose and water to pass, but not large polysaccharides like starch.

[edit] Conclusions

Diffusion is the movement of particles from an area of high concentration to an area of low concentration. One can see the effects of diffusion when one puts several balls tightly packed in a room and open the door to an adjacent room. The balls will gradually slide to the other room. Due to diffusion since the room had a high concentration of balls and the new room did not the balls diffused equally to both rooms. Another important piece of information to know about diffusion is that it is passive which means it requires no energy. Osmosis on the other hand is exactly like diffusion except that in osmosis the object that is being diffused is water, whereas diffusion implies no specific object. One example of osmosis can be seen in a plant when one pours water into a plant and the plant becomes turgid. This is because there is a lower concentration of water inside the plant so water diffuses into the plant. Osmosis like diffusion is also passive.